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. 2020 Jul 24;11:1114. doi: 10.3389/fphar.2020.01114

Figure 5.

Figure 5

Metformin protects chondrocytes against IL‐1β-induced apoptosis. Representative Western blots (A) and densitometric quantification (B) of BAX, Bcl2 and Cleaved Caspase-3 in cultured chondrocytes treated with IL-1β. Representative Western blots (C) and densitometric quantification (D) of BAX, Bcl2 and Cleaved Caspase-3 in chondrocytes incubated with or without metformin and IL-1β after 3-MA pretreatment. Representative Western blots (E) and densitometric quantification (F) in cultured chondrocytes when cells are incubated with or without metformin and IL-1β after ATG5 siRNA transfection. Expressions of target protein were normalized to GAPDH and shown as means ± SD. Significant differences between the treatment and control groups are indicated as *P<0.05 compared to group IL-1β; #P < 0.05 compared to the cells treated with IL-1β plus metformin(n=5). IL, Interleukin; BAX, BCL2(B-cell lymphoma 2) Associated X; 3-MA, 3-Methyladenine; ATG5, Autophagy related 5; siRNA, small interfering RNA; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; SD, standard deviation.