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. 2020 Jul 30;11(7):605. doi: 10.1038/s41419-020-02807-0

Fig. 4. Circ9119 targets miR-26a.

Fig. 4

a Bioinformatics analysis showing an miR-26a binding site in the circ9119 RNA sequence. b Dual luciferase reporter assay results after co-transfection of a luciferase reporter containing either wild-type (WT) or mutated (MU) circ9119, and an miR-26a mimic into HEK293T cells. c Fluorescence of circ9119 and miR-26a in HepG2 and Huh-7 cells. Green, circ9119; Red, miR-26a; Blue, DAPI. Scale bars: 10 μm. d qRT-PCR results showing miR-26a levels in HCC specimens (N = 20) and paired contiguous healthy controls (N = 20). e Correlation analysis between circ9119 and miR-26a expression in tumor tissues from 20 HCC cases (p < 0.001). f qRT-PCR analysis showing miR-26a expression in HHC and HCC cell lines, including SMMC-7721, HepG2, BEL-7404, and Huh-7. g, h qRT-PCR results showing miR-26a levels in all groups after Si-circ9119 or si-NC transfection in HepG2 and Huh-7 cell lines, respectively. *p < 0.05 vs. indicated groups.