Skip to main content
. 2020 Jul 30;11(7):605. doi: 10.1038/s41419-020-02807-0

Fig. 6. miR-26a targets the 3′-UTR of the JAK1 mRNA.

Fig. 6

a Bioinformatics analysis showing an miR-26a binding site in the JAK1 3′-UTR. b Dual luciferase reporter assay results following co-transfection of a luciferase reporter containing either wild-type (WT) or mutated (MU) JAK1 mRNA, and an miR-26a mimic into HEK293T cells. c qRT-PCR analysis showing JAK1 mRNA expression in HCC patients (N = 20) and healthy controls (N = 20). Correlation analysis between miR-26a and JAK1 mRNA expression in tumor tissues from 20 HCC cases (p < 0.001). d qRT-PCR showing JAK1 expression levels in HHCs and HCC cell lines (—). e–h HepG2 and Huh-7 cell lines transfected with both si-circ9119 and an miR-26a inhibitor (NC inhibitor) for 36 h. qRT-PCR and western blotting (WB) results showing JAK1 expression in each group. i–l HepG2 and Huh-7 cell lines after transfection with an miR-26a mimic or NC mimic. qRT-PCR and WB analyses showing JAK1 levels in each group. *p < 0.05 vs. indicated groups.