Table III.
Results of histology ten (Group C animals) and 12 (Group B animals) days after revision surgery in a porcine bone infection model. No revision surgery was done for Group A. Treatment Group B = limited debridement+ CERAMENT|G (CG). Treatment Group C = extensive debridement+ CG.
| Animal | Histology | PNA FISH/IHC positive bacteria within | ||||||
|---|---|---|---|---|---|---|---|---|
| Trabecular sequester | NG, n | Micro-abscesses | Fibrosis score | Mean PBA, μm (SD) | CERAMENT|G (CG) | Adjacent bone to CG | Distant located to CG | |
| A1* | Yes | 100 | Yes | 5 | 1385.84 (477.31) | N/A | N/A | Yes |
| A2† | No | 100 | Yes | 5 | 2132.83 (2018.26) | N/A | N/A | Yes |
| A3† | Yes | 100 | Yes | 4.4 | 1478.69 (1085.54) | N/A | N/A | Yes |
| B1 | Yes | 100 | Yes | 4.3 | 1424.84 (433.32) | Yes | Yes | Yes |
| B2 | Yes | 100 | Yes | 5 | 1851.9 (913.69) | Yes | Yes | Yes |
| B3‡ | No | 100 | Yes | 4.8 | 1372.83 (580.3) | Yes | Yes | Yes |
| C1 | No | 100 | No | 3.9 | 841.9 (348.97) | Yes | Yes | No |
| C2 | No | 100 | Yes | 3.6 | 889.63 (427.64) | Yes | Yes | No |
| C3§ | No | 100 | Yes | 5 | 2586.68 (1129.42) | Yes | Yes | No |
*
Pig euthanized seven days after inoculation.
†
Pig euthanized 19 days after inoculation.
‡
Pig euthanized four days after revision surgery.
§
Pig with unrevised bone lesion, thereby representing limited debridement/Group B.
IHC, immunohistochemistry; N/A, not applicable; NG, neutrophil granulocyte; PBA, pathological bone area; PNA FISH, peptide nucleic acid fluorescence in situ hybridization.