TABLE 2.

Revised Declaration of transparency and scientific rigour: checklist for information to be included in manuscripts, which report research that has used animals

BJP Item	Information to be provided	Where to include the information	ARRIVE 2.0 Item
1. Animal demographics	The source, species, strain, sex, range of age and/or weight of animals and any additional information that is relevant to the study.	Methods	Essential 8
2. Experimental procedures	Details of: anaesthesia, analgesia, antibiotics; surgical procedures; precautions to ensure asepsis; postsurgical analgesia (if there was recovery following surgery); any other post‐operative care; how the animal was killed	Methods	Essential 9
2. Experimental procedures	2b. Long term anaesthesia monitoring of vital signs, such as HR and blood gases or expired CO₂	Methods	Recommended
3. Justification for the validity of animal species or model selection	A scientific justification for the animal species and experimental model used in the study	Methods	Recommended 12
4. Ethical review	Details of the ethical review permissions, and national or institutional guidelines for the care and use of animals that authorised the research.	Methods	Recommended 14
5. Housing and husbandry	Details of: housing (type of facility e.g., specific pathogen free [SPF]); type of cage (eg open or IVC); bedding material; environmental enrichment; number of cage companions; configuration of cage‐mates (by littermates/genotype or mixed); bedding material; tank shape (for fish) etc.	Methods	Recommended 15
6. Animal welfare	Welfare‐related assessments, measurements and interventions (e.g., humane end‐points) that were carried out before, during, or (if applicable) after the experiment	Methods	Recommended 16