FIGURE 7.

Increases in early endosomes, Aβ and cognitive impairment are reversed by Dynasore and Rapamycin, respectively, in STZ‐induced DM mouse model. (a) Scheme of the experimental protocols in vivo. (b–h) The experimental mice were treated with vehicle, STZ, Dynasore, or Rapamycin, as described in Section 2. (b,e) Hippocampal slides for IHC were immunostained with Rab5‐specific antibody and counterstained with DAPI. Scale bars, 50 μm (magnification, ×200). n = 5 in each group. (c,g) Hippocampal Aβ 1–42 were measured by rat and mouse Aβ 1–42 specific ELISA assay. n = 5 in each group with two technical replicates each. (d,h) Mice were subjected to Y‐maze test to evaluate cognitive function. n = 6 in each group. (f) Hippocampal Rab5 and β‐actin were subjected to western blot. n = 5 in each group. *P < .05, significantly different from vehicle‐treated mice, ^# P < .05, significantly different from STZ‐treated mice. Quantitative data are presented as a mean ± SEM. All blots and immunofluorescence images are representative