FIGURE 2.

Astaxanthin (Ax) suppressed FFAs‐induced injury in hepatocytes. (a) CCK8 assay; (b) flow cytometry for apoptosis; (c) immunofluorescence for caspase 9 (×200); (d) relative expression of apoptotic mRNA; (e) expression of apoptotic proteins; (f) relative expressions of lipogenesis related genes in hepatocytes; (g) Oil Red O staining of hepatocytes (×200); (h) cellular TG levels. Data were presented as mean ± SEM; n = 5 in each group. ^# P<.05, significantly different from control, ⁺ P<.05, significantly different from FFA