TABLE 2.
Sequences of primer pairs used for amplification of mRNA by real‐time PCR
| For mice | For human | ||
|---|---|---|---|
| β‐actin | F GTGACGTTGACATCCGTAAAGA | β‐actin | F CTGGAACGGTGAAGGTGACA |
| R GCCGGACTCATCGTACTCC | R AAGGGACTTCCTGTAACAATGCA | ||
| BAX | F AGACAGGGGCCTTTTTGCTAC | BAX | F AAGAAGCTGAGCGAGTGT |
| R AATTCGCCGGAGACACTCG | R GGAGGAAGTCCAATGTC | ||
| IL‐1β | F CGATCGCGCAGGGCTGGGCGG | ─ | |
| R AGGAACTGACGGTACTGATGGA | |||
| iNOS | F CTCACTGGGACAGCACAGAA | ─ | |
| R GATGTGGCCTTGTGGTGAA | |||
| TNF‐α | F CAGGCGGTGCCTATGTCTC | ─ | |
| R CGATCACCCCGAAGTTCAGTAG | |||
| APOB | F GCTCAACTCAGGTTACCGTGA | APOB | F TGCTCCACTCACTTTACCGTC |
| R AGGGTGTACTGGCAAGTTTGG | R TAGCGTCCAGTGTGTACTGAC | ||
| ApoE | F CTCCCAAGTCACACAAGAACTG | ApoE | F GTTGCTGGTCACATTCCTGG |
| R CCAGCTCCTTTTTGTAAGCCTTT | R GCAGGTAATCCCAAAAGCGAC | ||
| ADRP | F CTTGTGTCCTCCGCTTATGTC | ADRP | F ATGGCATCCGTTGCAGTTGAT |
| R GCAGAGGTCACGGTCTTCAC | R GGACATGAGGTCATACGTGGAG | ||
| FAT/CD36 | F ATGGGCTGTGATCGGAACTG | FAT/CD36 | F GGCTGTGACCGGAACTGTG |
| R TTTGCCACGTCATCTGGGTTT | R AGGTCTCCAACTGGCATTAGAA | ||
| CPT1α | F TGGCATCATCACTGGTGTGTT | CPT1α | F TCCAGTTGGCTTATCGTGGTG |
| R GTCTAGGGTCCGATTGATCTTTG | R TCCAGAGTCCGATTGATTTTTGC | ||
| FATP5 | F TCTATGGCCTAAAGTTCAGGCG | FATP5 | F TGGAGGAGATCCTTCCCAAGC |
| R CTTGCCGCTCTAAAGCATCC | R TGGTCCCCGAGGTATAGATGAA | ||
| FGF21 | F GTGTCAAAGCCTCTAGGTTTCTT | FGF21 | F CTGTGGGTTTCTGTGCTGG |
| R GGTACACATTGTAACCGTCCTC | R CCGGCTTCAAGGCTTTCAG | ||
| FOXA1 | F ACATTCAAGCGCAGCTACCC | FOXA1 | F GCAATACTCGCCTTACGGCT |
| R TGCTGGTTCTGGCGGTAATAG | R TACACACCTTGGTAGTACGCC | ||
| PGC‐1a | F TATGGAGTGACATAGAGTGTGCT | PGC‐1a | F TCTGAGTCTGTATGGAGTGACAT |
| R GTCGCTACACCACTTCAATCC | R CCAAGTCGTTCACATCTAGTTCA | ||
| PPAR‐α | F AACATCGAGTGTCGAATATGTGG | PPAR‐α | F ATGGTGGACACGGAAAGCC |
| R CCGAATAGTTCGCCGAAAGAA | R CGATGGATTGCGAAATCTCTTGG | ||
| SREBP‐1c | F TTGTGGAGCTCAAAGACCTG | SREBP‐1c | F ACAGTGACTTCCCTGGCCTAT |
| R TGCAAGAAGCGGATGTAGTC | R GCATGGACGGGTACATCTTCAA | ||
| Tm6sf2 | F GATCACCTACGACCCTCTCTATG | Tm6sf2 | F GCATTGATGAGCGCCCTAATC |
| R TGGAGTGCAATGACAAGGTCC | R AGTGGGTCATAGGAGACCTCG | ||
| UCP1 | F GTGAACCCGACAACTTCCGAA | UCP1 | F AGGTCCAAGGTGAATGCCC |
| R TGCCAGGCAAGCTGAAACTC | R TTACCACAGCGGTGATTGTTC | ||
| Caspase 9 | F GGCTGTTAAACCCCTAGACCA | Caspase 9 | F CTTCGTTTCTGCGAACTAACAGG |
| R TGACGGGTCCAGCTTCACTA | R GCACCACTGGGGTAAGGTTT | ||
| Collagen I | F TTCACCTACAGCACGCTTGT | ─ | |
| R TCTTGGTGGTTTTGTATTCGATGA | |||
| ɑ‐SMA | F CCCAGACATCAGGGAGTAATGG | ─ | |
| R TCTATCGGATACTTCAGCGTCA | |||
| TGF‐β1 | F CCTGCAAGACCATCGACATG | ─ | |
| R GAGCCTTAGTTTGGACAGGATCTG | |||
| CTGF | F CGCCAACCGCAAGATTG | ─ | |
| R ACACGGACCCACCGAAGAC | |||