Skip to main content
. 2020 Jul 24;10:1225. doi: 10.3389/fonc.2020.01225

Figure 7.

Figure 7

DEN alters mitochondrial bioenergetics. (A) Downregulation of gene signatures of glycolysis, citrate cycle and OXPHOS defined by GSEA in THP1 cells after 24 h of exposure to DEN. (B) Oxygen consumption rate (OCR) profile plot obtained by the Seahorse Cell Mito Stress Test in the THP-1 cell (n = 4) after 24 h of exposure to increasing doses of DEN. (C) Basal respiration. (D) ATP-linked respiration. (E) Spare capacity. (F,G) Quantitative data of compensatory glycolysis and ratios of mitochondrial OCR to glycoPER calculated using Seahorse XF Glycolytic Rate assay (n = 3). (H) Measurement of glucose uptake in THP-1 cells after 24 h exposure to increasing doses of DEN (n = 5). Untreated cells (193,001 ± 44,454 RLU) were used as a reference and set as 1. Data are expressed as mean ± SEM. Statistical analysis was performed by using ANOVA followed by Dunnett's multiple comparison test with untreated group as control. *p < 0.05; **p < 0.01; ***p < 0.001. NES, normalized enrichment score; FDR, false discovery rate.