Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 24;10:1225. doi: 10.3389/fonc.2020.01225

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Salvestrini, Ciciarello, Pensato, Simonetti, Laginestra, Bruno, Pazzaglia, De Marchi, Forte, Orecchioni, Martinelli, Bertolini, Méndez-Ferrer, Adinolfi, Di Virgilio, Cavo and Curti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

DEN stimulation inhibits AML cell migration. Results of AML cell migration in transwell assays were shown as fold-change of the percentage of migration in comparison with the untreated condition set as 1. (A) Spontaneous migration of AML cells in presence of increasing doses of DEN in the upper chamber of transwell (n = 6). The percentage of migrating cells in control samples was 29.5 ± 6.3 SD. (B) Spontaneous migration of AML cells after a pre-treatment of 4 h with increasing doses of DEN (n = 8). The percentage of migrating cells in control samples was 34.3 ± 11.3 SD. (C) Migration of AML cells toward a gradient of DEN in the lower chamber of transwell (n = 5). The percentage of migrating cells in control samples was 32.0 ± 9.7 SD. (D) The histogram shows the effect of the presence of 0.1 mM DEN in the upper or the lower chamber on the CXCL-12 induced chemotaxis (150 ng/ml). The percentage of migrating cells in control samples was 34.1 ± 12.8 SD (n = 5). (E) CXCR4 expression analyzed by flow cytometer after overnight treatment with DEN (n = 6). The percentage of CXCR4⁺ cells in untreated samples was 23.6 ± 13.1 SD. Data are expressed as mean ± SD. Statistical analysis was performed by using ANOVA followed by Dunnett's multiple comparison test with untreated group as control and by Student's t-test. *p < 0.05; **p < 0.01; ***p < 0.001.