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. 2020 Jul 24;10:1240. doi: 10.3389/fonc.2020.01240

Table 1.

Probe sequences for p.L858R EGFR, exon 19 del EGFR, and p.E545K PIK3CA.

Mutation Probe sequence
EGFR L858R Capture probe 5′-TGG CCC GCC C-3′
Detector probe 5′-AAA ATC TGT GAT CTT GAC ATG CTG CGG TGT TTT GTG CAG-3′
EGFR exon 19 deletion Capture probe 5′-TGT TGC TTC CTT G-3′
Detector probe 5′-ATA GCG ACG GGA ATT TTA ACT TTC TCA CCT-3′
PIK3CA E545K Capture probe 5′-TCC TGC TTA GTG AT-3′
Detector probe 5′-TTC AGA GAG AGG ATC TCG TGT AGA AAT TGC TT-3′

The detector probes were biotinylated on the 3′ end.

HHS Vulnerability Disclosure