Table 1.
Mutation | Probe sequence | |
---|---|---|
EGFR L858R | Capture probe | 5′-TGG CCC GCC C-3′ |
Detector probe | 5′-AAA ATC TGT GAT CTT GAC ATG CTG CGG TGT TTT GTG CAG-3′ | |
EGFR exon 19 deletion | Capture probe | 5′-TGT TGC TTC CTT G-3′ |
Detector probe | 5′-ATA GCG ACG GGA ATT TTA ACT TTC TCA CCT-3′ | |
PIK3CA E545K | Capture probe | 5′-TCC TGC TTA GTG AT-3′ |
Detector probe | 5′-TTC AGA GAG AGG ATC TCG TGT AGA AAT TGC TT-3′ |
The detector probes were biotinylated on the 3′ end.