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. 2020 Jul 24;11:811. doi: 10.3389/fphys.2020.00811

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Copyright © 2020 Jensen, Bechshøft, Heisterberg, Schjerling, Andersen, Kjaer and Mackey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Method for identification of macrophages in the female study. Two triple positive cells (indicated with white arrows) located next to each other in a muscle section. CD68 (A) was used as a pan-macrophage marker, CD11b (B) as a proinflammatory phenotype marker and CD206 (C) as an anti-inflammatory phenotype marker. DAPI (D) identifies the cell nucleus. Cells were only marked positive for a marker if the cell nucleus was centrally located within an area of antibody staining. Scale bar indicates a length of 50 μm.