TABLE 3.
Antibodies.
Males | ||||
Host | Antibody | Company | Cat. no. | Concentration |
Primary antibody | ||||
Rabbit | CD68 | Sigma-Aldrich | HPA048982 | 1:200 |
Mouse | CD163 | Santa Cruz | SC-2006 | 1:200 |
Host | Company | Cat. no. | Concentration | |
Secondary antibody | ||||
Goat Anti-rabbit 568 | Thermo Scientific | A-11036 | 1:500 | |
Goat anti-mouse 488 | Thermo Scientific | A-1109 | 1:500 | |
Females | ||||
Host | Antibody | Company | Cat. no. | Concentration |
Primary antibody | ||||
Mouse | CD68 | Dako | M0718 | 1:500 |
Rabbit | CD11b | Abcam | AB52478 | 1:500 |
Goat | CD206 | RD Systems | AF2534 | 1:1000 |
Host | Company | Cat. no. | Concentration | |
Secondary antibody | ||||
Donkey anti mouse 488 | Abcam | Ab-150109 | 1:200 | |
Donkey anti rabbit 568 | Abcam | Ab-175693 | 1:200 | |
Donkey anti goat 680 | Thermo Scientific | A-21084 | 1:500 |