FIGURE 3.

Effects of MEG3 and let-7i overexpression on AS progression. (A) MEG3 expression was detected using qRT-PCR to assess the transfection efficiency of MEG3 overexpression plasmid. AS fibroblasts were transfected with pcDNA, MEG3, MEG3 + miR-con or MEG3 + let-7i, respectively. (B) The expression of let-7i was measured by qRT-PCR to evaluate transfection efficiency. (C–E) The mRNA expression levels of IL-1β, IL-6 and TNF-α in AS fibroblasts were determined by qRT-PCR. (F) WB analysis was used to test the protein levels of RUNX2, OCN and OPG in AS fibroblasts. All experiment required three biological replicates. *P < 0.05.