Table 2.
Specimens and Diagnostic Tests Associated With Each Study Visit for a Prospective Family Cohort Study in Kamphaeng Phet, Thailand, 2015–2019
| Specimen Collection | Diagnostic Testing | |||||||
|---|---|---|---|---|---|---|---|---|
| Study Visits | Serum | PBMC | Cord Blood | HLA | FlowNT 50 | HAI | DENV, ZIKV, JEV IgM, and IgG ELISA | DENV RT-PCR a |
| Enrollment | X | X | X | X | X | X | ||
| Annual follow-up | X | X | X | X | ||||
| Acute illness investigation | X | X | X | X | X | |||
| 14- and 28-day convalescentb | X | X | X | X | ||||
| 0-, 14-, and 28-day household contact evaluationb | X | X | X | X | X | |||
Abbreviations: DENV, dengue virus; ELISA, enzyme-linked immunosorbent assay; FlowNT, flow cytometry-based neutralization assay (50% titers); HAI, hemagglutination inhibition; HLA, human leukocyte antigens; IgG, immunoglobulin G; IgM, immunoglobulin M; JEV, Japanese encephalitis virus; PBMC, peripheral blood mononuclear cells; RT-PCR, reverse-transcriptase polymerase chain reaction; ZIKV, Zika virus.
a DENV RT-PCR is performed during an acute illness investigation (day 0), on the first day of a household investigation (day 0), and on acute specimens collected from household members who develop fever during the 28-day follow-up period.
b Collection of day-28 follow-up specimen is omitted for children younger than 5 years of age for both illness and household investigations, in order to limit the frequency of blood draws in very young children.