Figure 1.
Generation of CD123-Specific CAR T Cells
(A) Scheme of lentiviral vectors (LVs). Data throughout the figures are represented by the color code of the circles to the left of each construct. (B) Schematic for lentiviral transduction process. (C) Vector copy number (VCN) was determined by digital drop PCR analysis with primers within the lentiviral backbone (n = 5; p > 0.05 for all CAR T cell construct comparisons). (D–F) Flow cytometry evaluation was performed on day 8 after initial T cell activation. (D) CD123-CARCD20 expression (n = 5; p > 0.05). (E) CD20 transgene expression (n = 5; p > 0.05). (F) Coexpression of CD123-CAR and CD20. Representative histograms and dot plots are shown. The inset bar graph shows the mean and standard deviation values for cells expressing CD123-CAR and CD20 (n = 5; p > 0.05).