Figure 3.

CD123-CAR^CD20 T Cells Recognize and Kill CD123⁺ Targets in an Antigen-Specific Manner

(A) Effector cells were grown in cocultures with media, K562 (CD123^–), or Molm13 (CD123⁺) at an E:T ratio of 2:1 for 24 h. Supernatants were collected and evaluated for IFN-γ content by ELISA (n = 5; p < 0.0001 for NT versus CD123-CAR^CD20 T cell groups, and p > 0.05 for comparison among CD123-CAR^CD20 T cell groups). Scale magnification of data in (A; n = 5; p < 0.01 for comparison of 8.41BBz versus all other CD123-CAR^CD20 T cell groups). (B) Target cell populations were labeled with CFSE, incubated with effector T cells at the indicated ratios overnight and analyzed by flow cytometry by using absolute counting beads to determine cytotoxicity. n = 5; p > 0.05 for comparison on K562 targets and p < 0.0001 for CD123-CAR ^CD20, as compared with NT on Molm13.