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. 2020 Jun 27;21:636–655. doi: 10.1016/j.omtn.2020.06.024

Figure 2.

Figure 2

circHipk3 Regulates CM Proliferation In Vitro

(A) Detection of Ki67+ P0 CMs after transfection with si-circHipk3 or si-NC. Ki67+ CMs are indicated by arrows; n = 500 CMs, ∗p < 0.05, scale bars, 50 μm. (B) Detection of EdU+ P0 CMs after transfection with si-circHipk3 or si-NC. EdU+ CMs are indicated by arrows; n = 500 CMs, ∗p < 0.05, scale bars, 50 μm. (C) Detection of pH3+ P0 CMs after transfection with si-circHipk3 or si-NC. pH3+ CMs are indicated by arrows; n = 500 CMs, ∗p < 0.05, scale bars, 50 μm. (D) Detection of Aurora B+ P7 CMs after ADV-circHipk3 or ADV-vector infection; n = 500 CMs, ∗p < 0.05, scale bars, 50 μm. (E) Detection of Ki67+ P7 CMs after ADV-circHipk3 or ADV-vector infection. Ki67+ CMs are indicated by arrows; n = 500 CMs, ∗p < 0.05 versus, scale bars, 50 μm. (F) Detection of EdU+ P7 CMs after ADV-circHipk3 or ADV-vector infection. EdU+ CMs are indicated by arrows; n = 500 CMs, ∗p < 0.05, scale bars, 50 μm. (G) Detection of pH3+ P7 CMs after ADV-circHipk3 or ADV-vector infection. pH3+ CMs are indicated by arrows; n = 500 CMs, ∗p < 0.05, scale bars, 50 μm. (H) Quantification and representative images of 24 h time-lapse videos of P7 CMs transfected with ADV-circHipk3. ∗p < 0.05 versus; n = 250 CMs, scale bars, 50 μm. The arrows indicate the dividing CM. (I) Flow cytometry analysis of P7 CMs 48 h after ADV-circHipk3 or ADV-vector transfection; ∗p < 0.05 versus vector group in each stage.