Table 2.
Characteristics of serological tests relative to mouse bioassay and PCR, MC-qPCR (magnetic capture quantitative PCR), PD-qPCR (conventional quantitative PCR on acid pepsin-digested tissues)
| Serological test | % diagnostic sensitivity [95% CI] (positive/reference positivea) | % diagnostic specificity [95% CI] (negative/reference negativea) | Youden’s index |
|---|---|---|---|
| TgSAG1bio-BBMA | 93.5 [77.1–98.9] (29/31) | 100 [85.0–100] (28/28) | 0.94 |
| TgSAG1-ELISAbSH | 83.9 [65.5–93.9] (26/31) | 89.3 [70.6–97.2] (25/28) | 0.73 |
| TgSAG1-ELISAbSL | 96.8 [81.5–99.8] (30/31) | 60.7 [40.7–77.9] (17/28) | 0.58 |
| IFATb | 90.3 [73.1–97.5] (28/31) | 82.1 [62.4–93.2] (23/28) | 0.73 |
| MATb | 67.7 [48.5–82.7] (21/31) | 100 [85.0–100] (28/28) | 0.68 |
aChickens were regarded as reference-positive if at least one of the tissues (heart, drumstick) tested positive by one of the assays. The remaining chickens were regarded as reference-negative
bResults published previously [9], only shown for comparison
Abbreviation: CI, confidence interval