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. 2020 Jul 30;3:407. doi: 10.1038/s42003-020-01143-5

Fig. 3. Transcriptomic analysis of parasitism and grafting between P. japonicum and Arabidopsis.

Fig. 3

a Transcriptomic analysis was performed using RNA samples of the P. japonicum infected site and the P. japonicum graft site. For parasitism, total RNA was extracted before and 1, 2, 3, 5, and 7 days post infection (DPI). For grafting, total RNA was extracted before and 1, 2, 3, 4, 5, 6, 7, and 14 days after grafting (DAG) (with four biological replicates for each time point). Principal component analysis was performed from the obtained expression profile. Triangles and circles represent parasitism and grafting, respectively. PC, principal component. b Hierarchical clustering using Euclidean distance and Ward’s minimum variance method over ratio of RNA-seq data from five time points for P. japonicumArabidopsis parasitism and P. japonicum/Arabidopsis grafting against intact plants. Genes for which association with parasitism and grafting has been reported in previous studies are marked. Using the cDNA sequence of Arabidopsis as queries, tblastx was used to determine the most closely related homologs of P. japonicum. c Plots of the gene expression levels marked in b. Red background represents genes that behaved similarly in parasitism and grafting, and blue background indicates genes that behaved differently.