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. 2020 Jul 30;3:407. doi: 10.1038/s42003-020-01143-5

Fig. 7. Involvement of Glycosyl hydrolase 9B3 in establishment of parasitism of P. japonicum.

Fig. 7

a–d Expression patterns of PjGH9B3 promoter::GFP 1, 2, 3, and 4 days post infection (DPI). e RNAi targeting sequence for PjGH9B3 and the construct. f Relative expression levels of PjGH9B3 at 4 DPI (mean ± SE of three replicates). PjGH9B3-knockdown plants (PjGH9B3-RNAi) were categorized by the presence and absence of XB (XB and No XB, respectively). PjUBC2 was used as a reference gene. Representative images of the haustoria that did (g) and did not (h) form a xylem bridge (XB) at 4 DPI. i Ratio of the haustoria that did not form a XB (mean ± SE of four replicates, n = 4–26). Dots indicate each data point. An asterisk indicates statistical significance (Welch’s t-test, P < 0.05). VC, vector control. PjP. japonicum, AtArabidopsis, XB xylem bridge. Scale bars, 100 µm.