Figure 2.

Eosinophils Are Reduced in the Blood and Accumulate in the Heart Following Experimental Myocardial Infarction in Mice

(A) Representative flow cytometry plots showing the gating strategy applied to the left ventricle of wild-type BALB/c mice for detection of neutrophils (Ne), macrophages (Mo), and eosinophils (Eo). (B) Peripheral blood eosinophil count in BALB/c mice following MI (no MI: n = 12; other time points: n = 5 to 11 per group). (C) Total number of Siglec-F⁺Ly6G^int eosinophils detected by flow cytometry in the infarct and remote zones following MI (no MI: n = 3; other time points: n = 5 to 6 per group). (D) Representative histogram showing Siglec-F staining of splenic and infarct zone eosinophils at day 4 post-MI. (E) CD11b and Siglec-F median fluorescence intensity (MFI) of infarct and splenic eosinophils at day 4 post-MI (n = 4 per group). Median values with 25th and 75th percentiles are shown. ∗p < 0.05, ∗∗p < 0.01. Eo = eosinophil; MI = myocardial infarction; Mo = macrophage; Ne = neutrophil.