Skip to main content
. Author manuscript; available in PMC: 2020 Jul 31.
Published in final edited form as: Cell Rep. 2020 Jun 16;31(11):107764. doi: 10.1016/j.celrep.2020.107764

Figure 6. Concurrent Inhibition of Compensatory Signaling Enhances RAFi/ERKi Growth Inhibition.

Figure 6.

(A) Pa02C and Pa14C cells were treated with RAFi (0.3 μM), ERKi (0.04 μM), FRAX597 (PAKi, 1 μM), or MK2206 (AKTi, 0.6 μM) alone or in combination (slash indicates concurrent inhibition; plus sign indicates sequential inhibition, inhibitor addition after 72 h). Remaining cells were stained with crystal violet after a total of 5 days. Data are the mean average of two independent experiments. Error bars are shown as ± SEM.

(B) Pa02C and Pa14C cells were treated as in (A) for a total of 5 days. Cell lysates were immunoblotted to determine the levels of the indicated proteins. Data are representative of two independent experiments.