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. 2020 Jul 30;11(7):598. doi: 10.1038/s41419-020-02769-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The expression of LEF1 was identified in OA and healthy human cartilage tissues by q-PCR. b The LEF1 expression in chondrocytes treated with IL-1β was detected by q-PCR. c, d ChIP analysis of human chondrocytes treated with IL-1β using specific antibodies against LEF1 was shown. e Expression of RNF121 between OA and normal groups was identified by q-PCR. f RNF121 expression in LEF1-altered expressing chondrocytes was detected by western blot. g circRNA levels were identified by q-PCR. h CircRNF121 level was positively correlated with the modified Mankin’s scores. i Correlation analysis between the expression levels of LEF1 and circRNF121 was identified. j Exon2 and Exon3 region of RNF121 pre-mRNA was back- spliced and formed circRNF121. k CircRNF121 was amplified by divergent primers in cDNA but not in gDNA. GAPDH was used as a negative control. l Expression of circRNF121 was detected by q-PCR after transfection. Data were means ± SD of three independent assays (*P < 0.05).