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. 2020 Jul 30;11(7):598. doi: 10.1038/s41419-020-02769-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Three miRNAs were selected and analyzed by q-PCR. c The expression of miR-665 was negatively correlated with the modified Mankin’s scores. d The correlation analysis of the expressions of circRNF121 and miR-665 was shown. e The predicted binding sites between circRNF121 and miR-665 were presented. f The dual-reporter luciferase assay confirmed that circRNF121 was the direct target of miR-665. g RNA immunoprecipitation was performed in chondrocytes transfected with miR-NC and miR-665 mimic. CircRNF121 expression was detected by using qRT-PCR. RNA levels were presented as fold enrichment in Ago2 relative to IgG immunoprecipitates. h The localization of RNF121 and miR-665 was detected by FISH assay in human chondrocytes. i The predicted binding sites between MYD88 and miR-665 were shown. j The decreased luciferase was also shown to determine the direct binding of MYD88 and miR-665. k, l MYD88 expression was determined by western blot and IF staining in treated chondrocytes. Data were means ± SD of three independent assays (*P < 0.05).