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. 2020 Jul 30;11(7):602. doi: 10.1038/s41419-020-02812-3

Fig. 1. miR-1193 was identified from an automated, high-throughput screen as a specific synthetic lethal interacting partner with DNA-PKcs in M059J cells.

Fig. 1

a Workflow of the anti-miRNA screening procedure. GBM cells (M059J and U118MG) were seeded into seven 384-well plates in 50 μl of medium on the first day. In-house designed antisense oligonucleotides targeting 2590 human miRNAs were individually transfected to knockdown the corresponding miRNAs. Cells were fixed, and nuclei were stained with Hoechst on day 4. Automated nuclei counting was performed with an acumen® Cellista laser scanning imaging cytometer. b Z scores were calculated from the screen of all antisense oligonucleotides targeting 2590 human miRNAs in both U118MG cells and DNA-PKcs-deficient M059J cells. The Z scores for miR-1193 are indicated in red and identified by black arrows. c Effects of anti-miR-1193 on the proliferation of U118MG, M059J and several other cell lines. d The survival fractions were calculated. The data are presented as the mean ± SD values from triplicate biological experiments. *P < 0.05, **P < 0.01, ***P < 0.005. NS not significant: p > 0.05.