Fig. 6. miR-1193 inhibition in DNA-PKcs-deficient cells (M059J cells) results in activation of apoptosis through the ATR/Chk1/p53 axis and promotes chromosomal instability.

DNA-PKcs-proficient M059K cells and anti-miR-NC were used as controls. a Western blot of p-ATR, ATR, p-CHK1, CHK1, p-p53, p53, and GAPDH. Images of mitotic spread (b) and chromosomal abnormalities (d) were acquired and quantified. c, e The images are representative of three independent biological replicates. The data are presented as the mean ± SD values, and the error bars represent data from triplicate biological experiments. *P < 0.05, **P < 0.01, ***P < 0.005. NS not significant: p > 0.05.