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. 2020 Jul 30;10:12857. doi: 10.1038/s41598-020-69793-3

Table 2.

Representation of transcripts coding for families of secreted proteins in libraries of salivary glands from nymphs, females and males of R. microplus ticks according to

source of blood, whether genetically resistant or susceptible bovine hosts.

Library N° of reads Library N° of reads χ2 test
P value 		Library N° of reads Library N° of reads χ2 test
P value
Observed Expected Observed Expected Observed Expected Observed Expected
Mucins Glycine-rich cement-like proteins
NSGR **632 517 NSGS 522 636 45.89 < 0.001 NSGR 246 205 NSGS 213 253 14.06 < 0.001
FSGR 543 341 FSGS 230 431

212.52

 < 0.001

 FSGR 108 53 FSGS 12 67

100.72

 < 0.001
MSGR 15,946 13,463 MSGS 2,974 5,456

1669.40

 < 0.001

 MSGR 23,955 19,232 MSGS 2,839 7,561

4,412.028

 < 0.001
Lipocalins Kunitz-domain containing proteins
NSGR 508 1,158 NSGS 2,103 1,452 666.57 < 0.001 NSGR 69 294 NSGS 600 374 308.22 < 0.001
FSGR 1,213 2,163 FSGS 3,764 2,813

761.75

 < 0.001

 FSGR 394 633 FSGS 1,046 807

161.22

 < 0.001
MSGR 1,371 2012 MSGS 1,491 850

691.69

 < 0.001

 MSGR 1582 2,257 MSGS 1629 954

684.31

 < 0.001
Metalloproteases Serine Proteases
NSGR 240 393 NSGS 652 498

106.27

 < 0.001

 NSGR 109 49 NSGS 3 62 126.33 < 0.001
FSGR 585 499 FSGS 545 631

26.62

 < 0.001

 FSGR 15 7 FSGS 0 8

16.83

 < 0.001
MSGR 2,425 3,676 MSGS 2,817 1566

1,444.92

 < 0.001

 MSGR 3,671 3,769 MSGS 1679 1581

8.65

0.003†
Immunoglobulin G-binding proteins Trypsin-like inhibitor domain-containing proteins
NSGR 0 0 NSGS 0 0 N.S. * NSGR 29 29 NSGS 36 37 N.S
FSGR 0 0 FSGS 0 0 N.S FSGR 50 28 FSGS 13 35

30.40

 < 0.001
MSGR 2,251 1974 MSGS 548 824

131.80

 < 0.001

 MSGR 1,145 1,491 MSGS 973 627

271.330

 < 0.001
Antigen 5 Anti-microbial peptides
NSGR 78 236 NSGS 458 300 188.63 < 0.001 NSGR 220 290 NSGS 438 367 30.01 < 0.001
FSGR 13 7 FSGS 2 8

9.38

0.002†

 FSGR 205 110 FSGS 44 139

145.99

 < 0.001
MSGR 829 860 MSGS 392 360 N.S MSGR 848 1,058 MSGS 655 445

141.234

 < 0.001
Chemokine-binding evasins Serine carboxypeptidases
NSGR 11 73 NSGS 154 92 92.35 < 0.001 NSGR 3 7 NSGS 13 9 N.S
FSGR 78 91 FSGS 128 115 N.S FSGR 42 34 FSGS 35 43 N.S
MSGR 337 520 MSGS 402 219

217.84

 < 0.001

 MSGR 221 304 MSGS 210 127

75.30

 < 0.001
Transglutaminases Nucleotidases
NSGR 4 2 NSGS 4 2 N.S NSGR 41 74 NSGS 128 94 26.24 < 0.001
FSGR 13 6 FSGS 0 7

14.30

 < 0.001

 FSGR 141 126 FSGS 138 153

4.46

0.035†
MSGR 297 297 MSGS 124 124 N.S MSGR 235 377 MSGS 301 158

181.0

 < 0.001
DAP36 immunosuppressor Cystatins
NSGR 2 33 NSGS 73 42 50.61 < 0.001 NSGR 29 57 NSGS 101 73 24.21 < 0.001
FSGR 78 117 FSGS 187 148

22.43

 < 0.001

 FSGR 8 8 FSGS 10 10 N.S
MSGR 52 90 MSGS 76 37

53.35

 < 0.001

 MSGR 414 544 MSGS 358 228

104.20

 < 0.001
Serpins Kazal Domain-containing proteins
NSGR 156 92 NSGS 53 117 77.63 < 0.001 NSGR 65 52 NSGS 53 66

5.31

0.021†
FSGR 283 240 FSGS 57 100

25.98

 < 0.001

 FSGR 38 94 FSGS 96 40

112.134

 < 0.001
MSGR 121 186 MSGS 143 78

75.771

 < 0.001

 MSGR 11 36 MSGS 40 15

56.28

 < 0.001
Chimadanin thrombin inhibitors Digestive Carbohydrases
NSGR 16 86 NSGS 179 109

100.54

 < 0.001

 NSGR 430 223 NSGS 74 281 344.40 < 0.001
FSGR 34 100 FSGS 192 126

76.02

 < 0.001

 FSGR 7 4 FSGS 1 5

4.48

0.034†
MSGR 21 49 MSGS 49 21

53.16

 < 0.001

 MSGR 606 637 MSGS 298 267

4.96

0.026†
ML Domain-containing proteins Basic Tail superfamily proteins
NSGR 42 34 NSGS 36 44 N.S NSGR 17 46 NSGS 88 59 32.10 < 0.001
FSGR 42 21 FSGS 5 26

37.27

 < 0.001

 FSGR 8 8 FSGS 11 11 N.S
MSGR 90 115 MSGS 73 48

17.50

 < 0.001

 MSGR 323 467 MSGS 341 196

150.62

 < 0.001
Acid Tail superfamily proteins 8.9 KDA family proteins
NSGR 4 50 NSGS 109 63 73.84 < 0.001 NSGR 26 70 NSGS 132 88 47.93 < 0.001
FSGR 4 5 FSGS 7 6 N.S FSGR 47 51 FSGS 68 64 N.S
MSGR 91 192 MSGS 181 80

177.12

 < 0.001

 MSGR 36 114 MSGS 126 48

178.80

 < 0.001
9.4 KDA family proteins 10 KDA family proteins
NSGR 27 118 NSGS 240 149 123.81 < 0.001 NSGR 1,187 541 NSGS 33 679 1,388.86 < 0.001
FSGR 28 65 FSGS 120 83

36.96

 < 0.001

 FSGR 407 181 FSGS 2 228

505.98

 < 0.001
MSGR 4 18 MSGS 22 8

35.31

 < 0.001

 MSGR 4,751 3,497 MSGS 187 1,440

1,560.48

 < 0.001

†Not significant after Bonferroni correction; *N.S.: not significant; **: numbers in bold indicate that transcripts for the family are significantly overrepresented in the library.