Table 3.

Representation of transcripts coding for the main families of secreted proteins in salivary glands of nymphs, females and males of R. microplus ticks feeding on susceptible hosts.

Protein Family	N° of reads in Library						χ2 test; P value
	NSGS		FSGS		MSGS
	Observed	Expected*	Observed	Expected	Observed	Expected
Mucins	552	1627	230	955	2,974	1,174	4,063,636; < 0.001
Cement-like glycine-rich proteins	213	1,327	12	779	2,839	958	5,427,113; < 0.001
Lipocalins	2,103	3,187	3,764	1925	1,491	2,246	2,428,371; < 0.001
Kunitz-domain containing proteins	600	1,421	1,046	842	1629	1,013	907,726; < 0.001
Metalloproteases	652	1738	545	1,023	2,817	1,252	2,892,116; < 0.001
Serine proteases	3	730	0	429	1679	523	3,729,824; < 0.001
Immunoglobulin G-binding proteins	0	239	0	140	548	169	1,230,352; < 0.001
Trypsin inhibitor-like proteins	37	445	13	262	973	316	1978,723; < 0.001
Antigen 5 antioxidant enzymes	**458	372	2	218	392	262	298,833; < 0.001
Anti-microbial peptides	438	496	44	291	655	351	482,189; < 0.001
Chemokine-binding evasins	154	299	129	176	402	211	255,856; < 0.001
Serine carboxypeptidases	13	112	35	66	210	79	317,645; < 0.001
Transglutaminases	0	54	0	32	124	38	279,165; < 0.001
Nucleotidases	128	247	138	145	301	175	149,759; < 0.001
DAP36 immunosuppressors	73	147	187	86	76	103	161,951; < 0.001
Cystatins	101	204	10	120	358	144	469,574; < 0.001
Serpins	53	110	57	65	143	78	85,396; < 0.001
Kazal domain-containg proteins	53	82	96	48	40	58	62,749; < 0.001
Chimadanin thrombin inhibitor proteins	179	183	192	108	49	129	115,665; < 0.001
Digestive Carbohydrases	74	163	1	96	298	115	434,333; < 0.001
ML domain-containing proteins	36	50	5	29	73	35	64,956; < 0.001
Basic Tail superfamily	88	192	11	113	341	136	460,102; < 0.001
Acid Tail superfamily	109	130	7	76	181	91	154,033; < 0.001
8.9 KDA family	132	142	68	84	126	100	10,263; < 0.006†
9.4 KDA family	240	167	120	98	22	117	114,740; < 0.001
10 KDa family	33	99	2	57	187	68	301,372; < 0.001
Total reads in library	151.406		88.984		106.691

*: Data are for a 2 X 2 contingency table; **: numbers in bold indicate that transcripts for the family are significantly overrepresented in the library; †Not significant after Bonferroni correction.