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. 2020 Jul 30;10:12816. doi: 10.1038/s41598-020-68802-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heart regeneration is unaffected in cav1^cn100 mutants. (a–j) cav1^+/+ and cav1^cn100 hearts were cryoinjured and harvested 30 (a, b), 60 (d, e) or 90 dpci (g, h), and processed for AFOG staining, which labels collagen in blue, fibrin in red and healthy myocardium in brown. (i) heterozygous cav1^+/cn100 heart, 90 dpci. (c, f, j) Quantification of the injury site. Injuries were quantified as a percentage of the damaged tissue (collagen and fibrin) to the total area of the ventricle. 30 dpci n_WT = n_cn100 = 10; 60 dpci n_WT = n_cn100 = 10; 90 dpci n_WT = 9, n_cn100 = 12, t-test. Scale bars 250 μm.