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. 2020 Jul 30;11(7):604. doi: 10.1038/s41419-020-02804-3

Fig. 2. EN2 knockdown restrains the proliferation and migration of CRC cells.

Fig. 2

a, b KEGG pathway enrichment analyses of EN2 co-expression genes (a displays the genes in positive relation with EN2, while b displays those in negative relation). c The knockdown of EN2 in SW480 cells and the overexpression of EN2 in HCT8 cells was identified respectively by western blotting. d, f The capacity of cell proliferation was determined by CCK8 assay (d), cell clone-formation assay (e), and Edu assay (f). Scale bar: 50 µm. g The capacity of cell migration was assessed by transwell assay. Scale bar: 100 µm. h The capacity of cell migration was assessed by wound healing test. Scale bar: 0.5 mm. Data are presented as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.