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. 2020 Jul 30;11(7):604. doi: 10.1038/s41419-020-02804-3

Fig. 5. EN2 promotes cell proliferation and migration of CRC via activating CCL20 signaling.

Fig. 5

a The mRNA level of EN2 in SW480 cells transfected with vector, si-EN2 and si-EN2 + CCL20. b–e Cell proliferation and migration detected through CCK8 assay (b), Edu assay (c), transwell assay (d), and wound healing test (e) of SW480 cells transfected with vector, si-EN2, or si-EN2 + CCL20, respectively. b, c The capacity of cell proliferation determined by CCK8 assay (b) and cell clone-formation assay (c). d The capacity of cell migration assessed by transwell assay. Scale bar: 100 µm. e The capacity of cell migration assessed by wound healing test. Scale bar: 0.5 mm. Data are presented as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.