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. 2020 Jul 30;11:3807. doi: 10.1038/s41467-020-17647-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Model of full-length RNF4 displaying potential low-FRET and high-FRET states. b Single-molecule FRET histograms obtained from full-length RNF4 WT, labelled with donor/acceptor FRET dyes at SIMs and RING domain (residues 70,144; illustrated inset) (left). Pie charts indicate the percentage of molecules in a low-FRET (green) or high-FRET (red) state. Representative single-molecule trajectories obtained for RNF4 WT (right). c As in b but analysis performed on RNF4 ∆Basic. Single-molecule histograms were built from more than 500 molecules. Source data are provided as a Source Data file.