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. 2020 Jul 30;11(7):603. doi: 10.1038/s41419-020-02788-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Microvesicles in MSCs were analyzed by TEM. b Immunofluorescent staining of CD63 and CD9 in MSCs. c Differential centrifugation procedure for the isolation of exosomes from culture supernatants of MSCs (SN). d Isolated exosomes from control and IFN-γ-primed MSCs were analyzed by TEM. e Exosomes numbers isolated from control and IFN-γ-primed MSCs was analyzed by EXOCEP exosome quantitation kit. f The expression of CD81 and CD9 in exosomes from control and IFN-γ-primed MSCs were analyzed by western blotting. Scale bar a: 500 nm, b: 20 μm, and d: 100 nm. *P < 0.05, **P < 0.01, ***P < 0.001.