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. 2020 Jul 30;10:12856. doi: 10.1038/s41598-020-69774-6

Table 3.

Soluble (free and conjugated forms) phenolic acids (SPAs) of five different cereal fermented doughs individually inoculated with selected yeast strains.

Cereal flour Yeast strainsa SPAsb(mg/kg) Hydroxybenzoic acid (mg/kg) Vanillic acid (mg/kg) Syringic acid (mg/kg) p-coumaric acid (mg/kg) Ferulic acid (mg/kg) Sinapic acid (mg/kg)

Red-grained

common wheat

(cv. Aubusson)

Control 148b 5.71c 8.55b 3.82b 3.24b 89.5a 37.1c
ZEUS IBA 161a 7.89a 9.13a 4.82a 3.51a 91.7a 43.6a
D20Y 157a 7.33b 9.07a 4.66a 3.30b 92.1a 40.8b
D22Y 162a 7.60ab 9.08a 4.61a 3.42ab 95.7a 42.0ab
SEM 2 0.14 0.13 0.07 0.05 1.3 0.5
P(F) 0.004 < 0.001 0.033 < 0.001 0.019 0.053 < 0.001

Yellow-grained

common wheat

(cv. Bona vita)

Control 153b 7.46b 12.3b 9.04b 2.98a 82.2a 39.1b
ZEUS IBA 162a 10.14a 13.6a 11.09a 3.18a 80.1a 44.4a
L17Y 167a 10.30a 13.7a 11.73a 3.36a 83.3a 44.6a
L22Y 167a 10.03a 13.8a 11.41a 3.29a 83.7a 44.8a
SEM 2 0.11 0.2 0.25 0.12 1 0.6
P(F) 0.002 < 0.001 0.001 < 0.001 0.22 0.11 < 0.001

BLUE-grained

common wheat

(cv. Skorpion)

Control 146b 6.89c 11.3b 9.56b 3.2b 80.4a 35.1b
ZEUS IBA 162a 9.85ab 12.7a 12.5a 3.43ab 82.0a 41.2a
D8Y 159a 9.54b 12.7a 12.2a 3.39ab 79.6a 41.2a
L6Y 162a 9.96ab 12.9a 12.8a 3.59a 80.9a 41.4a
SEM 2 0.05 0.16 0.25 0.07 0.8 0.8
P(F) 0.002 < 0.001 < 0.001 < 0.001 0.023 0.266 0.001

Emmer

(cv. Giovanni Paolo)

Control 112c 5.33b 7.76b 2.53a 3.33c 54.4c 38.5c
ZEUS IBA 131b 7.09a 9.28a 2.94a 3.66b 63.4b 44.4ab
D18Y 135ab 7.03a 9.44a 2.83a 4.20a 67.6ab 43.5b
L22Y 141a 7.23a 9.27a 3.00a 3.97a 69.9a 48.1a
SEM 2 0.08 0.17 0.12 0.07 1.2 1
P(F) < 0.001 < 0.001 < 0.001 0.096 < 0.001 < 0.001 0.001

Hull-less barley

(cv. Rondo)

control 126c 3.00b 8.03b 1.53b 6.09ab 94.8b 12.1b
ZEUS IBA 140a 4.00a 9.99a 1.94a 6.55a 103a 13.8a
L10Y 130bc 3.92a 9.75a 1.88a 5.85bc 95b 13.7a
L15Y 133b 3.98a 9.91a 1.94a 5.34c 98.5b 13.4a
SEM 1 0.08 0.16 0.06 0.14 1 0.3
P(F) 0.001 < 0.001 < 0.001 0.006 0.002 0.001 0.006

Data are expressed on a dw basis. For each cereal type, means followed by different letters are significantly different, according to the Tukey’s HSD test (the ANOVA level of significance is shown in the table).

SEM standard error of the mean.

aControl, dough obtained from water and whole grain flour mixture, without yeast addition; ZEUS IBA, S. cerevisiae commercial baker’s yeasts.

bSum of the SPAs determined by means of the RP-HPLC/DAD.