Figure 1.

Expression of ASAP3 in Murine Somatosensory Cortex via IUE and Membrane Potential Recording with Random-Access, Single-Trial, and Single-Voxel Two-Photon Voltage Recording from Multiple Sites in Acute Slice

(A) Mouse brain at P8 after electroporation of pCAG-ASAP3b at E15.5.

(B) Representative two-photon single-plane image of ASAP3-expressing neurons in an acute cerebral cortical slice imaged by RAMP with the low magnification light path. Several neurons are seen in L2/3.

(C) Representative single-trial single-voxel voltage recording of spontaneous activity recorded from the two colored neurons simultaneously in (D). Top, raw (lighter colors) and filtered (50-Hz Butterworth low-pass filter; darker colors) optical traces. Bottom, the traces were corrected for the photobleaching and normalized to the baseline drift (black traces in top).

(D) Top, a two-photon image of ASAP3-expressing neurons with the high-magnification light path. Bottom, magnified views of the boxed regions in top. Colored crosses indicate selected voxels on the plasma membrane for voltage imaging in (C). Scale bar in A, 1 mm; B, 100 μm; C, 20 μm.

See also Figure S1.