Fig. 1.

Dual TLR7/NOD2L agonist stimulates both TLR7 and NOD2 and induces strong autophagy activity. (A) In vitro assessment of ligand-specific activation via the TLR7 and NOD2 pathways in HEK-Blue hTLR7 and hNOD2 cells. (B) Evaluation of the induction of autophagy in reporter cells, derived from HeLa cells, expressing a fluorescent GFP-LC3 fusion protein, after 8 hrs stimulation with 10 µM of ligands or 50 µM of Tamoxifen. Data are representative of 3 independent experiments. (C) Chemical structures and data sizing of the TLR7/NOD2L agonist. CL325 was solubilized as before at 4.2 mg/ml in acetone/buffer pH 9 and then diluted to PBS at 10 µg/ml. Nanosizer size measurement was possible. The CL325 is organized into particles with an average particle size of 300 nm +/- 70 nm. Even after sonication, the particle size remains the same, indicating a stable size.