Figure 2.

The correlation of detected AF with expected AF for mutations detected in four diluted samples

Shown in the plots is the high correlation of AF detected by SinoDuplex with expected AF for HD701M1 (A), HD701M2 (B), HD753M1 (C), and HD753M2 (D). The expected AF of mutations in the diluted samples is calculated as the detected AF in the undiluted samples multiplied by the diluted ratio. For sample HD753M2, as the expected AFs of most of the mutations are relatively low (<3%), an R² value of 0.595 is still considered a good correlation.