Table 2.

Validation of hotspot actionable mutations detected by SinoDuplex with ddPCR assay

Patient	Mutation	SinoDuplex AF	ddPCR AF
P1	EGFR p.T790M	0.3%	0.33%
P2	EGFR p.T790M	2.3%	1.82%
P3	EGFR p.T790M	0.1%	–
P4	EGFR p.L858R	0.3%	0.89%
P5	EGFR p.L858R	2.3%	4.6%
P6	KRAS p.G12D	2.0%	1.8%

Note: Due to low AF (0.1%), ddPCR failed to detect mutation EGFR p.T790M in Patient P3.