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. 2020 Jun 25;13(3):7. doi: 10.3892/mco.2020.2077

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Copyright: © Frames et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Bupivacaine induces apoptosis in patient-derived sarcoma tumor cells. Tumor cells were treated with 0.25 and 0.5% (8.66 and 17.33 mM, respectively) of bupivacaine for various time points (60, 120, 240 and 480 min). Cell death by apoptosis was analyzed using the Annexin V-fluorescein isothiocyanate (FITC) apoptosis kit after 24 h. The pseudocolor plot graphs represent Annexin-V/propidium iodide (PI) double staining to assess the number of apoptotic cells from both the patient-derived tumor cells and HTB-93 after treatment with 0.5% of bupivacaine at the indicated time points. Each experiment was performed in triplicate. ^***P<0.001, ^**P<0.01, ^*P<0.05 vs. control. SS, synovial sarcoma; UPS, undifferentiated pleomorphic sarcoma; OS, osteosarcoma.