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. 2020 Jul 24;11:1534. doi: 10.3389/fmicb.2020.01534

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Copyright © 2020 Manasian, Bustos, Pålsson, Håkansson, Peñarrieta, Nilsson and Linares-Pastén.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production and purification of the recombinant LrLyp (locus LGG_RS06710) and BlLyp, without signal peptides. SDS-PAGE is showing the molecular weight marker M (Lane 1), the cell extracts (CE) and the different chromatographic fractions (F1–F3) corresponding to each purified protein: LrLyp (Lanes 3–5) and BlLyp (Lanes 7–9).