FIGURE 2.

Multiple alignment of conserved blocks in lipolytic enzymes family II. Characterized enzymes were selected for the comparison with LrLyp from L. rhamnosus. Conserved catalytic amino acids are in bolds, S in the block I is the nucleophile, D and H in the block V are the donor proton and catalytic base respectively. G and N in the blocks II and III belong to the oxyanion hole. LrLyp belongs to clade I, since it lacks of block IIIa (Leščić Ašler et al., 2010). Where (*) denotes conserved or identical amino acid, (:) conservative or amino acid with similar biochemical property, (.) semiconservative and (_) non conservative amino acid.