. 2020 Jul 31;19:156. doi: 10.1186/s12934-020-01411-3

Table 1.

Mucic acid production characteristics of marine fungi in 24-well plates

Strain	T2	T3	D-161646	C1	C2	C3
Lactose
Mucic acid (g L⁻¹)	8.0 ± 0.2 ^aA	7.7 ± 0.0 ^aB	5.4 ± 0.2 ^bA
Yield mucic acid/d-galacturonic acid (g g⁻¹)	1.20 ± 0.05 ^a	1.13 ± 0.00 ^a	0.88 ± 0.05^b
d-Xylose
Mucic acid (g L⁻¹)	7.9 ± 0.3^aA	7.8 ± 0.2^aB	4.1 ± 0.1^bB	3.4 ± 0.2^aA	2.3 ± 0.1^bA	1.0 ± 0.0^cA
Yield mucic acid/d-galacturonic acid (g g⁻¹)	1.28 ± 0.05^a	1.29 ± 0.08^a	1.11 ± 0.18^a	0.50 ± 0.03^a	0.37 ± 0.01^b	0.14 ± 0.01^c
Glycerol
Mucic acid (g L⁻¹)	6.4 ± 0.2^aB	5.3 ± 0.3^bA	4.6 ± 0.1^cB	4.5 ± 0.2^aB	2.3 ± 0.1^bA	1.2 ± 0.6^cA
Yield mucic acid/d-galacturonic acid (g g⁻¹)	1.23 ± 0.02^a	0.98 ± 0.04^b	0.63 ± 0.02^c	0.57 ± 0.02^a	0.29 ± 0.02^b	0.16 ± 0.01^c
d-Glucose
Mucic acid (g L⁻¹)	6.5 ± 0.1^aB	6.6 ± 1.1^aAB	2.8 ± 0.2^bC	5.1 ± 0.4^aB	2.5 ± 0.2^bA	1.1 ± 0.1^cA
Yield mucic acid/d-galacturonic acid (g g⁻¹)	1.19 ± 0.01^a	1.24 ± 0.05^a	0.75 ± 0.09^b	0.82 ± 0.05^a	0.42 ± 0.02^b	0.17 ± 0.01^c

Marine Trichoderma sp. LF328 transformants (T2 and T3) and Coniochaeta sp. MF729 transformants (C1, C2 and C3) were grown in 24-well plates with d-galacturonic acid as substrate and lactose, d-xylose, glycerol or d-glucose as co-substrate. T. reesei Δgar1 udh strain D-161646 was used as a positive control. Values are mean ± standard error of the mean for n = 3 biological replicates. Values for the same specie in the same row with the same superscript letter (a to c) did not differ significantly (p > 0.05). Values for mucic acid concentration in the same column with the same superscript letter (A to C) did not differ significantly (p > 0.05)