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. 2020 Jul 24;10:379. doi: 10.3389/fcimb.2020.00379

Figure 1.

Figure 1

Glycoside hydrolases disperse P. aeruginosa and S. aureus biofilms. (A) P. aeruginosa PAO1 mono- and dual-species biofilms, and (B) S. aureus SA31 mono- and dual-species biofilms. (C) Example of in vitro well-plate model. Biofilms established in a 24-well cell culture plate for 48 h were treated with PBS (vehicle control), 5% amylase, 5% cellulase, or 10% GH containing both enzymes for 2 h. Percent dispersal was calculated as follows: (CFU in supernatant/ CFU in supernatant + CFU in biofilm). Two-way ANOVA was used to determine significance comparing enzyme to PBS #P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001. Two-way ANOVA was also used to determine significance comparing mono- and dual-species biofilm. *P < 0.05; **P < 0.01; n = 5.