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hBMSCs cultured in SHED-CM had less senescence and maintained stemness during long-term expansion. a Representative images of β-gal stained passage 3 (P3) and passage 8 (P8) hBMSCs cultured in DMEM, SHED-CM (P8-SHED-CM), and hBMSCs-CM (P8-hBMSCs-CM), and corresponding rate (%) of β-gal-positive cells per group. b Relative mRNA expression levels of senescence (p16 and p21) and stemness (Nanog and OCT4) markers per group. *P < 0.05, **P < 0.01, ***P < 0.001
