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. 2020 Jul 31;11:329. doi: 10.1186/s13287-020-01830-4

Table 1.

Primer sequences used in reverse transcription quantitative PCR (RT-qPCR)

Gene Sequence
p16 Forward: 5′-CCCCTTGCCTGGAAAGATAC-3′
Reverse: 5′-AGCCCCTCCTCTTTCTTCCT-3′
p21 Forward: 5′-AGCAGCGGAACAAGGAGT-3′
Reverse: 5′-TTACAGTCTAGGTGGAGAAACG-3′
Nanog Forward: 5′-AAGGCCTCAGCACCTACCTA-3′
Reverse: 5′-TGCACCAGGTCTGAGTGTTC-3′
OCT4 Forward: 5′-TGGATGTCAGGGCTCTTTGTC-3′
Reverse: 5′-ACCTTCCCAAATAGAACCCCC-3′
Runx2 Forward: 5′-TGGTTACTGTCATGGCGGGTA-3′
Reverse: 5′-TCTCAGATCGTTGAACCTTGCTA-3′
BSP Forward: 5′-GAACCACTTCCCCACCTTTTG-3′
Reverse: 5′-ATTCTGACCATCATAGCCATCG-3′
ALP Forward: 5′-TTCAAACCGAGATACAAGCACT-3′
Reverse: 5′-GGGCCAGACCAAAGATAGAG-3′
GAPDH Forward: 5′-GGAGCGAGATCCCTCCAAAAT-3′
Reverse: 5′-GGCTGTTGTCATACTTCTCATGG-3′