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. 2020 Jul 24;7:435. doi: 10.3389/fvets.2020.00435

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Copyright © 2020 Lu, Su, Du, Mo, Ke, Wang, Zhong, Yang, Chen, Wei, Huang, Liao and Ouyang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection and identification of two distinct bands of the ORF3 gene of PEDV in Guangxi. (A) Amplification of ORF3 gene in PEDV positive samples. M₁: DL 2000 marker; Lane 1: negative control; Lane 2: predicted product (740 bp); Lane 3: predicted product (740 bp) and large genomic deletion (~358 bp); Lane 4: CV777 strain; Lane 5: Zhejiang-08 strain; Lane 6: AJ1102 strain. (B) Identification of the plasmid, pMD-18T-ORF3, by enzyme digestion using PstI and BamHI. M₂: DL 5000 marker; Lane 1: The solid arrows indicate the predicted products (740 bp); Lane 2: The dashed arrows indicate products of PEDV variants with a large genomic deletion (~358 bp).