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. 2020 Jul 24;11:1099. doi: 10.3389/fphar.2020.01099

Figure 3.

Figure 3

Dioscin regulates SHP1 to reverse DHT-induced LNCaP-C81 cell proliferation and invasion. (A, B) DHT significantly reduces p-SHP1 (Y536) expression and increases subsequent expression of the p-Erk1/2 (T202/T204), p-P38 (T182) and p-P38 (T180) proteins (all p < 0.01). Dioscin reversed these effects of DHT on protein regulation in LNCaP-C81 cells (all p < 0.05). After inhibiting p-SHP1 (Y536) expression, DHT significantly promoted LNCaP-C81 cell proliferation (C), formation (F, G) and wound repair ability (H, I) (all p < 0.05). However, it had no significant effect on cell apoptosis (D, E) or migration (J, K) (all p > 0.05). Dioscin promoted p-SHP1 (Y536) expression and reversed DHT-induced LNCaP-C81 cell functions (all p < 0.05). Representative data from one of three independent experiments are shown in Figure 3 (A, D, F, H, J). * on behalf of p < 0.05; ** on behalf of p < 0.01.