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. Author manuscript; available in PMC: 2020 Jul 31.
Published in final edited form as: J Mol Cell Cardiol. 2019 May 7;132:49–59. doi: 10.1016/j.yjmcc.2019.05.002

Fig. 2.

Fig. 2.

Gsα deficiency induces SMC phenotype switch. A, Representative immunochemical staining of α-SMA, SMMHC, Vimentin and Osteopontin in abdominal aortas from control and GsαSMKO mice. IgG was labeled as the negative control. Scale bar, 50 μm. B, Quantification of IHC (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001 vs control. C, Western blot analysis of α-SMA, SMMHC, Osteopontin and Vimentin protein expression in control and GsαSMKO mice. D, Quantification of Western blot (n = 5). *P < 0.05, ***P < 0.001 vs control. E, Quantitative RT-PCR analysis of aortic α-SMA, SMMHC, Osteopontin and Vimentin mRNA levels in control and GsαSMKO mice (n = 5). *P < 0.05 vs control. Data are mean ± SEM.