Table 1. Linear epitopes present on spike (S) glycoprotein surface predicted through ElliPro in IEDB-analysis resource based upon solvent-accessibility and flexibility are shown with their antigenicity scores.
The highlighted green coloured regions were the potential antigenic domains while the yellow coloured region represents the trans-membrane domain of the S protein.
| No. | Chain | Start | End | Peptide | Residues | Score |
|---|---|---|---|---|---|---|
| 1 | A | 395 | 514 | VYADSFVIRGDEVRQIAPGQTGKIADYNYKLP DDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYG FQPTNGVGYQPYRVVVLS | 120 | 0.837 |
| 2 | 58 | 194 | FFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFAS TEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEF QFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYV SQPFLMDLEGKQGNFKNLREFVF | 137 | 0.835 | |
| 3 | 1067 | 1146 | YVPAQEKNFTTAPAICHDGKAHFPREGVFVSNG THWFVTQRNFYEPQIITTDNTF VSGNCDVVIGIVNNTVYDPLQPELD | 80 | 0.83 | |
| 4 | 201 | 270 | FKIYSKHTPINLVRDLPQGFSALEPLVDLPIG INITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYL | 70 | 0.76 | |
| 5 | 331 | 381 | NITNLCPFGEVFNATRFASVYAWNRKRISN CVADYSVLYNSASFSTFKCYG | 51 | 0.706 | |
| 6 | 700 | 720 | GAENSVAYSNNSIAIPTNFTI | 21 | 0.668 | |
| 7 | 27 | 35 | AYTNSFTRG | 9 | 0.66 | |
| 8 | 909 | 936 | IGVTQNVLYENQKLIANQFNSAIGKIQD | 28 | 0.633 | |
| 9 | 789 | 813 | YKTPPIKDFGGFNFSQILPDPSKPS | 25 | 0.6 | |
| 10 | 623 | 642 | AIHADQLTPTWRVYSTGSNV | 20 | 0.598 | |
| 11 | 891 | 907 | GAALQIPFAMQMAYRFN | 17 | 0.591 | |
| 12 | 579 | 583 | PQTLE | 5 | 0.551 | |
| 13 | 687 | 692 | VASQSI | 6 | 0.55 | |
| 14 | 653 | 659 | AEHVNNS | 7 | 0.539 | |
| 15 | 679 | 684 | NSPRRA | 6 | 0.521 | |
| 16 | B | 1067 | 1146 | YVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTH WFVTQRNFYEPQIITTDNTFVS GNCDVVIGIVNNTVYDPLQPELD | 80 | 0.826 |
| 17 | 89 | 194 | GVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATN VVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYS SANNCTFEYVSQPFLMDLEGKQGNFKNLREFVF | 106 | 0.816 | |
| 18 | 58 | 87 | FFSNVTWFHAIHVSGTNGTKRFDNPVLPFN | 30 | 0.81 | |
| 19 | 203 | 270 | IYSKHTPINLVRDLPQGFSALEPLVDLPIGINIT RFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYL | 68 | 0.748 | |
| 20 | 465 | 509 | ERDISTEIYQAGSTPCNGVEGF NCYFPLQSYGFQPTNGVGYQPYR | 45 | 0.727 | |
| 21 | 436 | 458 | WNSNNLDSKVGGNYNYLYRLFRK | 23 | 0.672 | |
| 22 | 700 | 720 | GAENSVAYSNNSIAIPTNFTI | 21 | 0.671 | |
| 23 | 27 | 35 | AYTNSFTRG | 9 | 0.666 | |
| 24 | 909 | 9036 | IGVTQNVLYENQKLIANQFNSAIGKIQD | 28 | 0.641 | |
| 25 | 624 | 643 | IHADQLTPTWRVYSTGSNVF | 20 | 0.617 | |
| 26 | 328 | 365 | RFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADY | 38 | 0.608 | |
| 27 | 891 | 907 | GAALQIPFAMQMAYRFN | 17 | 0.602 | |
| 28 | 577 | 583 | RDPQTLE | 7 | 0.598 | |
| 29 | 790 | 817 | KTPPIKDFGGFNFSQILPDPSKPSKRSF | 28 | 0.595 | |
| 30 | 673 | 693 | SYQTQTNSPRRARSVASQSII | 21 | 0.567 | |
| 31 | 526 | 537 | GPKKSTNLVKNK | 12 | 0.553 | |
| 32 | 653 | 661 | AEHVNNSYE | 9 | 0.548 | |
| 33 | 554 | 563 | ESNKKFLPFQ | 10 | 0.52 | |
| 34 | C | 56 | 194 | LPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFA STEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKV CEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFE YVSQPFLMDLEGKQGNFKNLREFVF | 139 | 0.84 |
| 35 | 1067 | 1146 | YVPAQEKNFTTAPAICHDGKAHFPREGVFV SNGTHWFVTQRNFYEPQIITTDNTFVSGN CDVVIGIVNNTVYDPLQPELD | 80 | 0.822 | |
| 36 | 201 | 270 | FKIYSKHTPINLVRDLPQGFSA LEPLVDLPIGINITRFQTLLALHR SYLTPGDSSSGWTAGAAAYYVGYL | 70 | 0.77 | |
| 37 | 27 | 35 | AYTNSFTRG | 9 | 0.676 | |
| 38 | 465 | 509 | ERDISTEIYQAGSTPCNGVEGFNCY FPLQSYGFQPTNGVGYQPYR | 45 | 0.675 | |
| 39 | 700 | 720 | GAENSVAYSNNSIAIPTNFTI | 21 | 0.658 | |
| 40 | 909 | 936 | IGVTQNVLYENQKLIANQFNSAIGKIQD | 28 | 0.633 | |
| 41 | 437 | 458 | NSNNLDSKVGGNYNYLYRLFRK | 22 | 0.629 | |
| 42 | 673 | 684 | SYQTQTNSPRRA | 12 | 0.619 | |
| 43 | 790 | 817 | KTPPIKDFGGFNFSQILPDPSKPSKRSF | 28 | 0.602 | |
| 44 | 891 | 907 | GAALQIPFAMQMAYRFN | 17 | 0.597 | |
| 45 | 578 | 583 | DPQTLE | 6 | 0.589 | |
| 46 | 620 | 631 | VPVAIHADQLTP | 12 | 0.579 | |
| 47 | 329 | 362 | FPNITNLCPFGEVFNATRFASVYAWNRKRISNCV | 34 | 0.571 | |
| 48 | 687 | 692 | VASQSI | 6 | 0.566 | |
| 49 | 835 | 845 | KQYGDCLGDIA | 11 | 0.567 | |
| 50 | 653 | 659 | AEHVNNS | 7 | 0.559 | |
| 51 | 527 | 536 | PKKSTNLVKN | 10 | 0.546 | |
| 52 | 635 | 642 | VYSTGSNV | 8 | 0.51 |